Adjuvants in PCR Reactions
Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.
Attotron Biosensor Corporation
R & D company for development of biosensors and related products for the research and educational markets.
Biosource molecular methods booklet
56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).
Cleaved Amplified Polymorphic Sequence
Illustration of principle. CAPS corresponds to RFLP. From Corvallis Forestry Research Community, Corvallis, USA.
Design Oligonunucleotide Primers
Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the Universit…
Dolan DNA Learning Centers Gene Almanac
Educational site on topics in genetics and gene expression from Cold Spring Harbor Laboratory, USA.
FISH Guide and Troubleshooting
Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, ma…
GeneOhm Sciences
Tests on gruup B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.
General Notes on Primer Design in PCR
Article on the influence of the design of the primer on the outcome of PCR, by Dr Vincent R. Prezioso, published by Eppendorf, North America.
Ingenetix GmbH
Develops technology and products for DNA and mRNA research. Also provide DNA testing for the determination of parentage/paternity and custom DNA sequencing, oligonucleotide synthes…
Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation
Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
Life at High Temperatures
Article by TD Brock from the web site of Department of Bacteriology, University of Wisconsin - Madison on the thermophilic organisms in Yellowstone hot springs, including Thermus a…
MJ Research
Manufactures and sells products for molecular biology including thermal cyclers and sequencers / genotypers.
Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
Basic discussion of the optimization of PCR, from the web site of Brinkmann Instruments.
PCR Amplification of cDNA Segments by 2 Stage Nested PCR
Protocol from the method database of NIH, USA.
PCR animated
Animation illustrating the principle of PCR, from the University of Ghent, Belgium.
PCR Protocols
Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Tox…
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